ASP.NET部署错误

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访问网站提示：Server Application Unavailable
The web application you are attempting to access on this web server is currently unavailable. Please hit the “Refresh” button in your web browser to retry your request.
Administrator Note: An error message detailing the cause of this specific request failure can be found in the application event log of the web server. Please review this log entry to discover what caused this error to occur.
服务器上有其他ASP.NET网站，且运行正常。安装了.net 1.1、2.0、4.0。开始怀疑共用同一个程序池的原因，分配单独地址池，情况依旧。


查看事件查看器，发现应用程序下很多asp.net的报错，每访问一次网站就会记录很多同样的报错。
有一个报错内容是：
Error:Failed to execute request because the App-Domain could not be created. Error: 0×80070005 Access is denied.
查看Event Viewer,看到的错误为:
Error:Failed to execute request because the App-Domain could not be created. Error: 0×80070005 Access is denied.
Warning:Failed to initialize the AppDomain:/LM/W3SVC/1052609887/Root/skyy_mobile
Exception: System.IO.FileLoadException
Message: Could not load file or assembly ‘System.Web, Version=2.0.0.0, Culture=neutral, PublicKeyToken=b03f5f7f11d50a3a’ or one of its dependencies. Access is denied.
StackTrace: at System.Reflection.Assembly.nLoad(AssemblyName fileName, String codeBase, Evidence assemblySecurity, Assembly locationHint, StackCrawlMark& stackMark, Boolean throwOnFileNotFound, Boolean forIntrospection)
at System.Reflection.Assembly.InternalLoad(AssemblyName assemblyRef, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection)
at System.Reflection.Assembly.InternalLoad(String assemblyString, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection)
at System.Activator.CreateInstance(String assemblyName, String typeName, Boolean ignoreCase, BindingFlags bindingAttr, Binder binder, Object[] args, CultureInfo culture, Object[] activationAttributes, Evidence securityInfo, StackCrawlMark& stackMark)
at System.Activator.CreateInstance(String assemblyName, String typeName)
at System.AppDomain.CreateInstance(String assemblyName, String typeName)
at System.AppDomain.CreateInstance(String assemblyName, String typeName)
at System.Web.Hosting.ApplicationManager.CreateAppDomainWithHostingEnvironment(String appId, IApplicationHost appHost, HostingEnvironmentParameters hostingParameters)
at System.Web.Hosting.ApplicationManager.CreateAppDomainWithHostingEnvironmentAndReportErrors(String appId, IApplicationHost appHost, HostingEnvironmentParameters hostingParameters)

解决办法：在该网站目录权限下设置
ASP.NET、IWAM_MachineName、Local_Service、Network_Service、System
设置后,一切运行正常。
我的是添加了Network_Service之后解决的。